Determination of lactoferrin in goat milk by HPLC method

The aim of this study was the determination of lactoferrin in goat milk using HPLC method and the report of the concentration of lactoferrin in goat milk.Milk samples were collected at a goat farm in the Harbin Region, China.It were established raw milk ( n = 24) and pasteurized milk ( n = 24) that were collected during lactation.Lactoferrin contents were analyzed by reverse phase high- performance liquid chromatography ( RP- HPLC) with diode- array detector PDA 2996. Detection was carried out at the wavelength 210 nm. The average recovery, the concentration range of calibration curve, the limit of detection and the limit of quantification was 94.2%, 50~300μg /mL, 12μg /mL, 40μg /mL separately.The average concentration of lactoferrin in goat milk was 122.1μg /mL.The average concentration of lactoferrin in pasteurization ( at 72℃ for 30s) goat milk was 103.9μg /mL.The heat treatment resulted in no significant effect on the lactoferrin content.No statistically significant differences ( p < 0.05) were found between the values of raw and pasteurized goat milk.