Research of the optimization of extraction process of Euphausia pacifica phospholipid and analysis of fatty acids composition

The effects of ethanol concentration, the ratios of solvent to krill sample, extraction temperature and extraction time on the yield and purity of phospholipids extracted from Euphausia pacifica was investigated.Judging from the results of single factor experiment and orthogonal experiment, the optimal processing conditions were as follows: 95% ethanol, 6 to 1 ratio of solvent to krill sample, a 4h extraction time and a 40℃ extraction temperature.Under such conditions, the reasonable yield of 17.58% and purity of 46.09% were obtained.The purity reached 78.85% with a further purification by acetone at 4℃.The fatty acids of the further purified phospholipids were then analysed by GC.Results showed that the unsaturated fatty acids were 52.46%, the total percentage of EPA and DHA were 24.51% among all the fatty acids analysed.