Optimization of ultrasonic-assisted extraction of total triterpenes from Matteuccia struthiopteris by response surface methodology

Matteuccia struthiopteris was regarded as study material. According to the center combination ( Box-Benhnken) experimental principles, four factors and three levels which were based on single factor experiments were selected to design response surface test. The response factor was the yield of triterpenes, independent variables were including ethanol concentration, solid- liquid ratio, ultrasonic temperature, and ultrasonic time. By means of regression analysis, the results showed that the optimum technological method to extract triterpenes from Matteuccia struthiopteris were as follows: ethanol concentration 60%, ratio of solid- liquid 1 ∶ 35, ultrasonic temperature 63℃ and ultrasonic time 39 min. The actual value was 36.0304 mg /g, compared with the theoretical value ( 35.7263 mg /g) , the RSD was 0.40%.From this study, it could be concluded that the extraction system was reliable and practical, the certain reference and theoretical guidance for further research can be provided.