Simultaneous determination of uronic acids, amino sugars and neutral sugars in polysaccharide from sea cucumber

TIAN Xin; SHENG Wen-jing; ZHAO Yuan-yuan; DONG Jun; XUE Yong; XUE Chang-hu

doi:10.13386/j.issn1002-0306.2014.15.009

TIAN Xin, SHENG Wen-jing, ZHAO Yuan-yuan, DONG Jun, XUE Yong, XUE Chang-hu. Simultaneous determination of uronic acids, amino sugars and neutral sugars in polysaccharide from sea cucumber[J]. Science and Technology of Food Industry, 2014, (15): 85-89. DOI: 10.13386/j.issn1002-0306.2014.15.009

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Simultaneous determination of uronic acids, amino sugars and neutral sugars in polysaccharide from sea cucumber

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A high performance liquid chromatographic ( HPLC) method for simultaneous determination of uronic acids, amino sugars and neutral sugars in polysaccharide from sea cucumber by pre- column derivatization with1-phenyl-3-methyl-5-pyrazolone ( PMP) was developed. The polysaccharide was hydrolyzed by the 2mol /L trifluoroacetic acid, and then derivatived with PMP. The uronic acids, amino sugars and neutral sugars were separated well by reverse- phase HPLC. The results showed that glycosaminoglycan consisted of mannose, galactose, fucose, glucosamine, galactosamine, glucuronic acid.The HPLC method is simple, rapid, sensitive, and convenient.

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Science and Technology of Food Industry

Simultaneous determination of uronic acids, amino sugars and neutral sugars in polysaccharide from sea cucumber

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