Comparison of content, composition and antioxidant activity of germinated oat phenols by different extraction methods

Oat seeds were steeped and germinated in this article.Phenol compounds from non-germination oat and germinated oat were extracted by solvent extraction method and enzyme assistant extraction method respectively.The total phenol content, composition and antioxidant activity of extracts by different extraction methods were analyzed.The results indicated that enzyme assistant extraction method was more beneficial to the extraction of oat phenols than solvent extraction method.There existed some differences between enzymatic extracts and solovent extracts on the relative content of avenanthramides 2c, 2p, 2f, t2 and t3.Meanwhile, new component was detected in enzymatic extracts, which might result in decrease of antioxidant activity.In addition, germination could be used as an effective method to increase oat phenol content and antioxidant activity.