Study on the high cell density culture of Lactococcus lactis

FENG Hui-jie; MU Wan-meng; ZHANG Tao; JIANG Bo

doi:10.13386/j.issn1002-0306.2014.14.035

FENG Hui-jie, MU Wan-meng, ZHANG Tao, JIANG Bo. Study on the high cell density culture of Lactococcus lactis[J]. Science and Technology of Food Industry, 2014, (14): 197-201. DOI: 10.13386/j.issn1002-0306.2014.14.035

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Study on the high cell density culture of Lactococcus lactis

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In order to improve the cell density of Lactococcus lactis SYFS1.009, the fermentation culture medium and fed-batch culture conditions were studied. The optimum fermentation conditions by single-factor experiment and orthogonal experiment were determined:glucose 30g/L, yeast extract 40g/L, K2HPO440g/L, MgSO40.6g/L, MnSO4·H2O 0.1g/L, Tween 80 0.5mL/L. After cultivated in this medium for 14h at 30℃, DCW (dry cell weight) was 3.1g/L, which was about 3.4 times higher than in MRS (DCW 0.9g/L) . After 12h culture, the DCW was up to7.3g/L and OD600was 15.3.

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Science and Technology of Food Industry

Study on the high cell density culture of Lactococcus lactis

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