Analysis of organic acids in pickled radish by reverse phase high performance liquid chromatography

WANG Ran; LI Xiao-lin; LI Min; WEI Jing; CHEN An-jun; AO Xiao-lin; PU Biao

doi:10.13386/j.issn1002-0306.2014.13.053

WANG Ran, LI Xiao-lin, LI Min, WEI Jing, CHEN An-jun, AO Xiao-lin, PU Biao. Analysis of organic acids in pickled radish by reverse phase high performance liquid chromatography[J]. Science and Technology of Food Industry, 2014, (13): 283-287. DOI: 10.13386/j.issn1002-0306.2014.13.053

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Analysis of organic acids in pickled radish by reverse phase high performance liquid chromatography

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A method for simultaneous determination of eight organic acids in pickled radish by reverse phase high performance liquid chromatography was established. YMC- Pack ODS- AQ C18column ( 5μm, 120A, 4.6mm ×150mm) was used at 27℃ and mobile phase was 4% CH3OH- 0.05 mol /L KH2PO4 ( pH2.9) with flow rate of0.6mL /min. Detection wavelength was 210nm.The extraction with ultrapure water was better.With these conditions, eight organic acids in pickled radish could be effectively separated and detected.The recoveries were 90.59% to103.85%, and the relative standard deviations were 0.34% to 2.18%.

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Science and Technology of Food Industry

Analysis of organic acids in pickled radish by reverse phase high performance liquid chromatography

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