The impact of ultrasound combined with CMC-Na on the extraction of heme from horse blood

DAI Wen-ting; TAO Yong-xia; YANG Hai-yan; BAI Yu-jia; FENG Zuo-shan

doi:10.13386/j.issn1002-0306.2014.13.047

DAI Wen-ting, TAO Yong-xia, YANG Hai-yan, BAI Yu-jia, FENG Zuo-shan. The impact of ultrasound combined with CMC-Na on the extraction of heme from horse blood[J]. Science and Technology of Food Industry, 2014, (13): 257-261. DOI: 10.13386/j.issn1002-0306.2014.13.047

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The impact of ultrasound combined with CMC-Na on the extraction of heme from horse blood

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Heme was extracted from horse blood, using ultrasound method combined with CMC-Na. Based on the single factor experiment, the Box- Behnken design and response surface method were used to set up the mathematical model and determine the best extraction conditions.As a result, the optimum process conditions were obtained as following: ultrasonic power was 338W, ultrasonic time was 10min, deionized water content was 5 times the size of red blood cells ( RBC) , and extraction pH was 5.5.Under these conditions, the yield of heme reached maximum 0.42g /50mL which was in good agreement with the predicted value.

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The impact of ultrasound combined with CMC-Na on the extraction of heme from horse blood

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