Optimization of the preparation of collagen antioxidant peptide of Phascolosoma esculenta by response surface method

The preparation conditions of Phascolosoma esculenta antioxidant peptide were optimized. Papain was optimum enzyme selected by enzyme filtering experiment.Hydrolysis time, enzyme amount and ratio of material to liquid were determined by single factor experiment as independent variables, while total antioxidant capacity ( TAOC) resistance as the response value.The optimum solutions for Phascolosoma esculenta antioxidant peptide by response surface method were listed as follows: reaction time was 4.28h, and enzyme dosage was 3614U /g, and solid to liquid ratio was 1 ∶36. Taking operability into account, the extraction conditions were adjusted to: reaction time was 4.25h, and enzyme dosage was 3600U /g, and liquid ratio was 1 ∶ 36. Under such conditions, the corresponding T-AOC of the Phascolosoma esculenta collagen peptide was 3.8U /mg.