Purification of the hepatoprotective fraction from Hippophae rhamnoides L.subsp.turkestanica

Objective: To establish the purification craft of the hepatoprotective fraction from Hippophae rhamnoides L.subsp.turkestanica. Method: The adsorption and desoption ability of the total triterpenoids and phenolics were carried out to select the appropriate marcoporous resin.Then, the dynamic adsorption and desoption experiments were used to study the appropriate purification craft.Result: AB-8 resin was better than other resins.The optimum purification conditions: sample solution concentration 0.067g /mL, 9¹0BV sample solution in pH6, flow rate 2BV/h, Elution solvent 70%, ethanol washing 3.5BV.Under these optimum conditions, the purified product contained total triterpenoids 49.3% and total phenolic 36.1%, the total effective fraction content was 85.4% 。