Cloning and bioinformatics analysis of Cu /Zn-SOD gene from cantaloupe

MA Wei-rong; SHAN Chun-hui; TONG Jun-mao; LIU Tao

doi:10.13386/j.issn1002-0306.2014.13.031

MA Wei-rong, SHAN Chun-hui, TONG Jun-mao, LIU Tao. Cloning and bioinformatics analysis of Cu /Zn-SOD gene from cantaloupe[J]. Science and Technology of Food Industry, 2014, (13): 181-185. DOI: 10.13386/j.issn1002-0306.2014.13.031

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Cloning and bioinformatics analysis of Cu /Zn-SOD gene from cantaloupe

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A superoxide dismutase gene was cloned by the Cu /Zn-superoxide dismutase gene ( Cu /Zn-SOD) from cucumber and watermelon has cloned in NCBI, which was named HmCu /Zn-SOD.The length cDNA of HmCu /Zn-SOD was 403bp.The characteristics were analyzed and predicted by the tools of bioinformatics.The results showed that HmCu/Zn-SOD was a hydrophilic, non-transmembrane and non-secretory protein.The main motif of Cu/ZnSOD in secondary structure was random coils and extended strands.Despite sequence variations, the structure and the composition of amino acid sequences of HmCu/Zn-SOD were highly similar to that of other green plants.

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Science and Technology of Food Industry

Cloning and bioinformatics analysis of Cu /Zn-SOD gene from cantaloupe

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