Fabrication of organophosphorus pesticide biosensor modified by polythionine

DING Jian-ying; ZHOU Yong-tao; WANG Jia-xu; CHEN Wen-fei; CHEN Meng-lin

doi:10.13386/j.issn1002-0306.2014.12.006

DING Jian-ying, ZHOU Yong-tao, WANG Jia-xu, CHEN Wen-fei, CHEN Meng-lin. Fabrication of organophosphorus pesticide biosensor modified by polythionine[J]. Science and Technology of Food Industry, 2014, (12): 74-76. DOI: 10.13386/j.issn1002-0306.2014.12.006

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Fabrication of organophosphorus pesticide biosensor modified by polythionine

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An amperometric biosensor for the detection of organophosphorus pesticides in vegetables was developed. This novel bienzyme electode biosensor was fabricated with acetylcholinesterase (ACHE) immobilized by chitosansilica on the surface of the polythionine modified glassy carbon electrode. Under optimal experimental conditions. The inhibition rate versus the logarithm of dimethoate concentration was linear over the range of0.1μg/mL to 60μg/mL with the linear correlation coefficient of 0.9961. The detection limitation for dimethoate, calculated by inhibition rate of 10%, was 0.05μg/mL.

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Science and Technology of Food Industry

Fabrication of organophosphorus pesticide biosensor modified by polythionine

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