Identification of wort turbid substances in Jiangsu Daner Barley wort

Identification of wort turbid substances in Jiangsu Daner barley wort was performed.Results indicated that carbohydrates were the most abundant portion in wort turbid substances and the content of carbohydrates was82.57%, followed by proteins and polyphenols.Ion exchange chromatography was used to study the carbohydrates.It showed that the most abundant hydrolyzed monosaccharide was glucose, followed by galactose, arabinose and xylose.SDS- PAGE and MALDI- TOF/TOF were used to identify the protein components of wort turbid substances.Results revealed that the molecular weights of proteins were mainly 25 ~ 45ku and less than18.4ku and most of these proteins were derived from water-soluble proteins and ethanol-soluble proteins of malt.Mass spectrometry analysis showed that the maximum amount of haze protein was chain A of AMY2 /BASI complex, in addition to a small amount of protein Z4, γ3 hordein, caleosin 2 and BTI- CMe2.1.The results were instructive for the improvement of the quality of Jiangsu barley malt.