Development of colloidal-gold Immunochromatographic strip for rapid and quantitative detection of olaquindox in pig liver

The aim of the present study was to develop colloidal gold immunochromatography assays for rapid and quantitative detection of olaquindox ( OLA) residues in pig liver.Prepared the colloidal gold immunochromatography test strips and evaluated the specificity, a quantitative method based on T/C ratio was developed to detect OLA residue in pig liver.Preparation of the test strip could be accomplished qualitatively as well as quantitatively detection in 15min, and there was little cross- reactivity detected with carbadox, mequindox and 3- methyl-quinoxaline-2-hydroxy acids.When the pig liver had OLA concentration at a range of 1 to 200ng /mL, the test strip showed a higher linear relationship, and the limit of detection ( LOD) of immunochromatographic strip for OLA was 6.83ng /mL.For negative liver samples, the recoveries rate was 90.9% ¹05.0% at 25, 50 and 100ng /mL.