Simultaneous determination of cyclic adenosine monophosphate and apigenin and quercetin in Fructus jujuba by HPCE

High performance capillary electrophoresis ( CE) was developed for the determination of cyclic adenosine phosphate, apigenin and quercetin in jujube.The effects of several factors such as concentration of running buffer and the pH, concentration of the additive β- cyclodextrin, the detection wavelength and the separation voltage for separating result were investigated.In optimum condition, three groups chemical component had been achieved separation detection.A good linearity between peak area ratio of the common peak to the concentration was found in the range of 16.5⁵29.4mg /L for cyclic adenosine phosphate, 5.5¹76.4mg /L for apigenin and 8.8²82.4mg /L for quercetin, and correlation coefficients ( r) were 0.9998, 0.9998, 0.9997, respectively.The average recoveries were 97.31%, 95.62% and 94.88% for cyclic adenosine phosphate, apigenin and quercetin, respectively.The retention time of relative standard deviation was 6.7%, 5.1% and 5.3% and the peak area of relative standard deviation was 4.5%, 5.8% and 4.2%.The results showed that the method was simple, rapid and with satisfactory recoveries and good reproducibilities.It can be used to detection of elements of cyclic adenosine phosphate, apigenin and quercetin.