Optimization of ultrasonic extraction of total flavonoids from Sarcopyramis nepalensis Wall using response surface methodology

To optimize extraction yield of total flavonoids from Sarcopyramis nepalensis Wall using ultrasonic extraction method, the study was designed and carried using response surface methodology. On the basis of single-factor experiments, extraction conditions of the extraction yield of total flavonoids from S. nepalensis Wall were optimized by response surface analysis method. Results showed that the optimum extraction conditions were as following:extraction temperature 56℃, ultrasonic power 870W, ultrasonic time 51min. The confirmatory test value of extraction yield of total flavonoids was 2.53mg/g, the relative standard deviation was0.87% with predicted value (2.55mg/g) . Results could provide a reference for the total flavonoids extraction from S. nepalensis Wall.