Study on purification of total saponins from Acanthopanx obouatus Hoo

Objective:Inquiry into the optimal purification condition of total saponins from Acanthopanx obouatus Hoo with macroporous adsorptive resins. Methods:Screen the optimal macroporous resin by the static test on five different models macroporus resin. Optimize the technological parameter by investigating the absorption and desorption performances of the optimal macroporous resin on total saponins from Acanthopanx obouatus Hoo. Results:LS-303 macroporous resin was the optimal macroporous resin on total saponins from Acanthopanx obouatus Hoo. Optimum technology was that:the concentration of sample solution was 3mg/mL with loading amount of 9BV, the velocity of sample loading was 2BV/h, the eluent was 80% alcohol, the elution velocity was2BV/h, the elution volume was 2.5BV. Conclusion:LS-303 macroporous resin in purifying total saponins from Acanthopanx obouatus Hoo and the advantages of the developed method for industrial application, liked simple operation, safety, and low cost.