Study on condition of polymerization of 11S globulin by TGase

The conditions of polymerization of 11S globulins by Transglutaminase (TGase) was studied. Firstly, the 11S globulins was hydrolyzed by Alcalase and then polymerized by TGase, the result showed that soybean11S globulins polymer molecular weight about 15ku. The appropriate enzyme amount for polymerization of 11S was 30U/g, over or below was not good for the reaction. Ionic strength was 0.2, that was beneficial to the formation of polymer. The pH between 8.0⁹.0 was better for polymerization. The appropriate temperature was45℃, that was good for TGase playing a role in polymerzation. Emulsification of native was 30.27% and stability was 37.78%, after modification the emulsification was 61.15% and stability was 79.28%.