JIAN Hua-li, TIAN Wen-xiang, YANG You-hui, LIAO Mei-de. Study on the molecular structure of polysaccharide produced by PS04 strain[J]. Science and Technology of Food Industry, 2014, (08): 114-117. DOI: 10.13386/j.issn1002-0306.2014.08.016
Citation: JIAN Hua-li, TIAN Wen-xiang, YANG You-hui, LIAO Mei-de. Study on the molecular structure of polysaccharide produced by PS04 strain[J]. Science and Technology of Food Industry, 2014, (08): 114-117. DOI: 10.13386/j.issn1002-0306.2014.08.016

Study on the molecular structure of polysaccharide produced by PS04 strain

  • A single component peak was found in PS04 polysaccharide purity analysis using S-200 HR filtration of chromatography. The weight mean molecular weight of the PS04 polysaccharides examined by GPC was4009ku, the peak positions molecular weight was 3174ku, and distribution width index was 1.348.The structure of PS04 polysaccharide was identified by infrared spectral, HPLC, and NMR. The results showed that PS04polysaccharide was a typical polysaccharide compounds and the main composition was fructose and glucose (proportion 7∶1) . NMR analysis demonstrated that there were furan nucleus and the chain was linked by β-2, 6and β-1, 2 linkage, so PS04 polysaccharide was similar to levan.
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