Specific PCR detection method of apple juice andpeach juice species

SUN Jian-xia; BAI Wei-bin; CAO Chun-ting; ZHANG Zhen-hua; QIU Rui-xia; WU Xi-yang; OU Shi-yi

doi:10.13386/j.issn1002-0306.2014.07.060

SUN Jian-xia, BAI Wei-bin, CAO Chun-ting, ZHANG Zhen-hua, QIU Rui-xia, WU Xi-yang, OU Shi-yi. Specific PCR detection method of apple juice andpeach juice species[J]. Science and Technology of Food Industry, 2014, (07): 288-291. DOI: 10.13386/j.issn1002-0306.2014.07.060

Citation:

Specific PCR detection method of apple juice andpeach juice species

Graphical Abstract

Abstract

Abstract

It used mal d 4.02 gene for apple allergen and MA023a gene for peach microsatellite mark as specific primers of apple and peach, respectively. The chloroplast AccD gene of highly conserved plant was used as endogenous gene. Species-specificity of apple and peach fruit juice was identified by PCR technology. In the results, the specific PCR detection method could accurately identify apple and peach species. The minimum detection limit of the specific PCR method for apple and peach fruit was 5ng /mL and 10ng /μL.This method could qualitative identify apple and peach juice quickly and accurately.

FullText(HTML)

References (15)

Supplements (0)

Cited By

Science and Technology of Food Industry

Specific PCR detection method of apple juice andpeach juice species

Abstract

Catalog

Export File

Citation

Format

Content