Optimization of culture media of Mucor mihei producing milk-clotting enzyme through solid-state-fermentation

LI Xue-peng; SHI Xi-xong; FENG Rui-zhang; ZHAO Wei; ZHANG Yan; LIANG Qi; ZHANG Wei-bing

doi:10.13386/j.issn1002-0306.2014.07.021

LI Xue-peng, SHI Xi-xong, FENG Rui-zhang, ZHAO Wei, ZHANG Yan, LIANG Qi, ZHANG Wei-bing. Optimization of culture media of Mucor mihei producing milk-clotting enzyme through solid-state-fermentation[J]. Science and Technology of Food Industry, 2014, (07): 130-133. DOI: 10.13386/j.issn1002-0306.2014.07.021

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Optimization of culture media of Mucor mihei producing milk-clotting enzyme through solid-state-fermentation

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The culture medium was optimized to improve the yield of milk-clotting enzyme from Mucor miehei.The results of the single factor experiment showed that the best carbon source, the optimal nitrogen source, and the best enzyme inducer were glucose, ammonium nitrate, and whey powder, respectively.On this basis, through the orthogonal experimental design, the optimal extraneous components we obtained were as follows:2.5% glucose, 1.0% ammonium nitrate, and 2.0% whey powder. In the optimized medium, milk-clotting activity of Mucor miehei reached ( 3649.52 ± 3.62) SU /mL and increased by 54.5%.

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Science and Technology of Food Industry

Optimization of culture media of Mucor mihei producing milk-clotting enzyme through solid-state-fermentation

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