Study on purification of recombinant antihypertensive peptides VLPVPR with macroporous adsorption resin

LI Yan; SUN Hai-yan; ZHOU Li-zhen; LIU Dong

doi:10.13386/j.issn1002-0306.2014.07.015

LI Yan, SUN Hai-yan, ZHOU Li-zhen, LIU Dong. Study on purification of recombinant antihypertensive peptides VLPVPR with macroporous adsorption resin[J]. Science and Technology of Food Industry, 2014, (07): 284-288. DOI: 10.13386/j.issn1002-0306.2014.07.015

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Study on purification of recombinant antihypertensive peptides VLPVPR with macroporous adsorption resin

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To separate and purify peptide VLPVPR, the absorption capability and purification effect of VLPVPR on macroporous adsorption resin ( DA201-C Ⅱ, D4020 and HPD100B) were evaluated. Adsorption and desorption experiments were carried out to screen the suitable macroporous adsorption resin for VLPVPR.DA201-CⅡ was the best macroporous adsorption resin for purification of VLPVPR.The optimization conditions were pH9.8, feeding rate 2mL /min, feeding volume 100mL, ethanol concertration 70%, elute rate 2.7mL /min and elute volume 75mL. The recovery ratio was 96.9% and the purity ratio was 27.4%.

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Science and Technology of Food Industry

Study on purification of recombinant antihypertensive peptides VLPVPR with macroporous adsorption resin

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