Fingerprints study on Pinus koraiensis polyphenols by HPLC

To establish the HPLC fingerprint of Pinus koraiensis polyphenols. The optimal chromatographic separating condition was:reverse C 18 column ( 200mm × 4.6mm, 5μm) , the mobile phase was 0.05% trifluoroaceticacid ( A) -methanol ( B) , elution gradient:0¹1min, 5% →15% B; 11¹6min, 15% →17% B; 16²3min, 17% →23% B; 23³6min, 23% →35% B; 36⁵0min, 35% →40% B; 50⁶5min, 40% →50% B; 65⁷0min, 50% →5% B; flow rate:0.8mL /min, the detection wavelength: ( 280nm) , this method satisfied the methodological study of the HPLC fingerprint.Based on the analysis and comparison of 8 batches of samples, eatablishing the HPLC fingerprint of Pinus koraiensis polyphenols. Marked 14 stable common peaks and determined coffee acid as the reference peak.It was concluded that this method was simple, accurate, reproducible and provided an effective method for the quality control standard of Pinus koraiensis polyphenol.