Influence of bitter melon saponin on the glucose consumption of insulin resistance HepG2 cell in vitro

High concentration insulin was added into HepG2 cells to induce glucose metabolism abnormal.The optimal conditions of insulin resistant HepG2 cells ( HepG2 /IR) model was investigated from insulin dose and effect time.HepG2 cells were divided into control group, insulin resistant group, pioglitazone hydrochloride group, bitter melon saponin groups ( 100, 300, 500, 700μg /mL) . The glucose consumption was measured by glucose oxidase method ( GOD) .The cell activity was estimated by MTT method.These results showed that treatment with 30μg /mL insulin for 36h was the best condition for induction of HepG2 /IR model.Compared with HepG2 /IR group, treatment with 300μg /mL bitter melon saponin not only could alleviate insulin resistant HepG2 cells significantly, but cell activity was increased.