Study on the process optimization of extraction of polysaccharide of silkworm pupa and decolorization

Objective:Optimize the extraction technic of polysaccharide of silkworm pupa and study the condition of decolorizes the polysaccharide. Methods:By means of orthogonal experiment, optimize the extraction technic of polysaccharide used microwave method according to the yield index of polysaccharide. Explored the decolorizing effect of polysaccharide by using the ion exchange resin (D202, D113) and macroporous adsorptive resin (HZ-841, HZ-806, HZ-803) and found the optimum conditions of the decolorization of polysaccharide by orthogonal experiment. Results:The optimum conditions of the traditional extraction technic were extraction 3.5h at 90℃、ratio of solid to liquid 1∶25, the yield index of polysaccharide was 3.95%;the optimum conditions of the microwave extraction of polysaccharide were microwave power 600W, extraction time 9min, ratio of solid to liquid 1 ∶25, the yield index of polysaccharide was 4.49%. The optimum conditions of the decolorization of polysaccharide was HZ-807 Resin、pH4.0、temperature 45℃. Conclusion:It was significantly increased of the yield index of polysaccharide by using the optimized microwave extraction method, and the process was simple、reasonable and feasible. In the most appropriate experimental condition, the decolorization rate of polysaccharide of silkworm pupa, polysaccharide retention rate and protein removal rate was 88.56%, 62.15%, 92.21% with macroporous resin by static absorbing test. In the most appropriate experimental condition, the decolorization rate of polysaccharide of silkworm pupa, polysaccharide retention rate and protein removal rate was 89.43%, 65.58%, 90.56% with macroporous resin by dynamic test test.