Optimization of heterotrophic culture conditions of Chlorella sp. for lutein production

WANG Shu-hui; LIN He-tong; WANG Liang; CAO Li-li

doi:10.13386/j.issn1002-0306.2014.04.062

WANG Shu-hui, LIN He-tong, WANG Liang, CAO Li-li. Optimization of heterotrophic culture conditions of Chlorella sp. for lutein production[J]. Science and Technology of Food Industry, 2014, (04): 179-183. DOI: 10.13386/j.issn1002-0306.2014.04.062

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Optimization of heterotrophic culture conditions of Chlorella sp. for lutein production

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The effect of carbon source, nitrogen source, pH as well as temperature on the growth of a self-isolated Chlorella sp. and the production of lutein were investigated. Results showed that glucose was the best carbon source and potassium nitrate was the best nitrogen source to support sustained and rapid growth of this strain of Chlorella sp. as well as lutein production. Results of optimization were as follows:the glucose concentration was 20g·L-1, potassium nitrate concentration was 1.25g·L-1, initial pH was 6.0 and temperature was 30℃. In this condition the maximal lutein concentration was 12.27mg·L-1.

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Science and Technology of Food Industry

Optimization of heterotrophic culture conditions of Chlorella sp. for lutein production

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