Production of acid α-Amylase Aspergillus Niger strains by mutagenesis of protoplast

HUANG Wei; LIU Yong-le; WANG Fa-xiang; YU Jian; LI Xiang-hong; WANG Jian-hui; LI Yan

doi:10.13386/j.issn1002-0306.2014.03.026

HUANG Wei, LIU Yong-le, WANG Fa-xiang, YU Jian, LI Xiang-hong, WANG Jian-hui, LI Yan. Production of acid α-Amylase Aspergillus Niger strains by mutagenesis of protoplast[J]. Science and Technology of Food Industry, 2014, (03): 160-162. DOI: 10.13386/j.issn1002-0306.2014.03.026

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Production of acid α-Amylase Aspergillus Niger strains by mutagenesis of protoplast

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Aspergillus Niger 0-2-1 was used as original strain.This strain of separation and purification of spores protoplast was treated by Ultra Violet ( UV) . The mutant with the bigger ratio of hydrolyzation circle diameter to bacterial colony diameter was screened so as to assay acid α- amylase activities. After genetic stability testing, three strains ( UV9, UV17, UV31) with an high activity of acidα-amylase were screened.Strain UV9, UV17 and UV31 were obtained with an acid α- amylase activity of 184.66, 162.73 and 167.31U /mL.The activities obtained were 54.9%, 38.1% and 42.0% higher than that of the parent strain.

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Production of acid α-Amylase Aspergillus Niger strains by mutagenesis of protoplast

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