Optimization of Schizothorax Prenanti Tchang protein enzymatic hydrolysis technology by response surface methodology

Response Surface Methodology ( RSM) was used to optimize the process that fish protein from Schizothorax Prenanti Tchang (SPT) was hydrolyzed by the animal proteases hydrolysate enzymic that screened through various protease hydrolysis of fish protein. According to Box-Benhnken center combination experiment design principle on foundation of signal factors experiment, selected four significant parameters, including hydrolysis time, hydrolysis temperature, enzyme concentration, solid-fluid ratio for optimization of RSM. Established a mathematic model of hydrolysis degree of fish protein from SPT, and combined with the regression analysis the influence of various and interactions. The result of SPT flesh hydrolysis showed that the optimum conditions were as follows:hydrolysis time was 4.5h, hydrolysis temperature was 54 degree, enzyme concentration was 5080U/g, solid to liquid of ratio was 1∶3.2 (g/mL) , pH8.0. Under this conditions, the degree of hydrolysis was 13.67%, compared with the theoretical predicted value of 13.33%, about 2.55% absolute vaule difference. It was indicated that SPT hydrolysis technology with response surface methodology had greatly significant.