Optimization of composite enzymatic hydrolysis assisted ultrasonic for extraction of favonoids compounds from hawthorn

Total flavonoids from hawthorn were extracted by composite enzyme hydrolysis synergism ultrasonic. Optimized the extraction conditions of flavonoids with single factor experiment and orthogonal test, the optimal conditions of the single factor of was composite enzyme concentration:3% cellulose and 4% pectinase, pH6, enzymolysis temperature:60℃, enzymolysis time:1.5h, ethanol concentration:70%, solid-liquid ratio:1∶40g/mL. 40℃ ultrasonic extract 30min, results showed that quantity of extract total flavonoids was 85.5mg/g under such conditions, comparing with ultrasonic extraction, the extraction ratio by enzymatic hydrolysis synergism ultrasonic was 46.1% higher, and 27.6% higher than enzymactic hydrolysis method.