Study on purification and characterization of superoxide dismutase in Trametes versicolor mycelium

HUANG Zhi-li; HUANG Yan-jun; ZHANG Li-jun

doi:10.13386/j.issn1002-0306.2014.02.018

HUANG Zhi-li, HUANG Yan-jun, ZHANG Li-jun. Study on purification and characterization of superoxide dismutase in Trametes versicolor mycelium[J]. Science and Technology of Food Industry, 2014, (02): 120-123. DOI: 10.13386/j.issn1002-0306.2014.02.018

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Study on purification and characterization of superoxide dismutase in Trametes versicolor mycelium

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Superoxide dismutase ( SOD) was extracted from Trametes versicolor mycelium with membrane filtration, ammonium sulfate fractionation and SephadexG-75 gel column chromatography. The SOD was purified 12.6-fold with a specific activity of 4.61U/mg protein and a yield of 50.6%. The ultraviolet spectrum of purified SOD showed an absorbance peak at 257nm. The activity of enzyme was stable under 50℃ but decreased with higher temperature. Meanwhile, it was sensitive to chloroform-enthanol but inhibited obviously by H2 O2. These results indicated that the SOD from Trametes versicolor mycelium was Cu, Zn-SOD with a good thermostability.

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Science and Technology of Food Industry

Study on purification and characterization of superoxide dismutase in Trametes versicolor mycelium

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