Optimization of production conditions of protein feed fermented scallop processed disposal by response surface methodology

The processed scallop disposal was utilized as raw material to ferment. The conditions of producing protein feed by compound culture of yeast and lactic acid bacteria were studied. The effects of additive amount of water, time, temperature, inoculum size and adding amount of glucose on the content of protein and amino nitrogen were explored. The fermentation conditions were optimized by Box-Behnken experiment design and ridge analysis, and the result indicated that the optimum conditions were the proportion of yeast, Lactobacillus plantarum and Lactobacillus bulgaricus 3 ∶ 1 ∶ 1, nature moisture content of culture medium, fermentation temperature 31.4℃, time 79h, inoculum 18.9% and the accession amount of glucose 21.9%. Under the optimized conditions, the content of protein was 64. 01 % which increased 11. 92 % than unfermented raw material.