Optimization of cellulase-assisted extraction of osthole from root of Radix Angelica Pubescens using response surface methodology

ZHAO Mei-rong; LI Xue-hu; LIU Yao-ming; YAO Hai-chao; LU Xi-hong; ZHOU Xiang; XIN Zhi-jun; SE Zhu; LIANG Jian-ping

doi:10.13386/j.issn1002-0306.2014.01.032

ZHAO Mei-rong, LI Xue-hu, LIU Yao-ming, YAO Hai-chao, LU Xi-hong, ZHOU Xiang, XIN Zhi-jun, SE Zhu, LIANG Jian-ping. Optimization of cellulase-assisted extraction of osthole from root of Radix Angelica Pubescens using response surface methodology[J]. Science and Technology of Food Industry, 2014, (01): 206-209. DOI: 10.13386/j.issn1002-0306.2014.01.032

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Optimization of cellulase-assisted extraction of osthole from root of Radix Angelica Pubescens using response surface methodology

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Response surface methodology ( RSM) was applied to optimize the extraction conditions of osthole.Based on single factor experiment, pH, the amount of cellulase enzymolysis temperature and time were chosen as influencing factors, the yield of osthole was selected as response value.The mathematical model was established by Box- Behnken central composite design.The results showed that the optimal extraction conditions were as follows: pH5.0, the amount of cellulase 3%, enzymolysis temperature 50℃, enzymolysis time 60min.In this optimal process condition, the yield of osthole was 0.926%.

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Optimization of cellulase-assisted extraction of osthole from root of Radix Angelica Pubescens using response surface methodology

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