A preliminary attempt to establish the high performance liquid chromatographic fingerprint of kang zhuan tea

The chromatographic fingerprint of kang zhuan tea was established by high performance liquid chromatography (HPLC) .The HPLC conditions and extraction condition of kang zhuan tea were optimized.According to the average relative retention time, common peaks, and average relative peak area in HPLC were assayed respectively.The results showed that the components of kang zhuan tea could be well separated by using a gradient elution with acetonitrile and water containing 0.3% formic acid as the mobile phase at a flow rate of 1mL/min, a detection wavelength of 280nm and the column temperature of 35℃.The chromatographic fingerprint was analyzed based on the analysis of HPLC chromatogram of 48 batches of kang zhuan tea samples.It was confirmed that 34 peaks in chromatograms could be selected as characrerixation peaks.Results from a cluster analysis of the average relative peak area had shown that the kang zhuan tea samples of eight production enterprises could be divided into about three types.The similarity of 48 batches of kang zhuan tea was more than 0.90.The research supplied a reference method to the identification and quality control of kang zhuan tea.