HPLC determination of ergothioneine in mushrooms of different species

Samples were extracted with water by heating reflux and the extract was filtration.The supernatant was then analyzed by NH 2 column with elution (acetomitrile- 5mmol /L ammonium acetate solution (80 ∶ 20) as mobile phase) and quantified by external standard method.A good linearity was shown in the range of 0.005⁰.106mg /mL.The average recoveries at three spike levels (0.011, 0.014, 0.017mg /mL) were 102.0%, 100.2% and 101.5%, respectively.The contents of ergothioneine in mushrooms at three different concentration were in the range of 0.000% ⁰.280%.The method was simple and reliable, and might be used for the determination of ergothioneine in the mushrooms of different species and the foundation for the application of rare amino acids.