Optimization of activation condition of prothrombin from horse blood by using response surface methodology

Mihriban·Hojahmat; Mawjuda·Yarmanmat; Reziye·Kari; Rehman·Ala

doi:10.13386/j.issn1002-0306.2013.22.061

Mihriban·Hojahmat, Mawjuda·Yarmanmat, Reziye·Kari, Rehman·Ala. Optimization of activation condition of prothrombin from horse blood by using response surface methodology[J]. Science and Technology of Food Industry, 2013, (22): 153-156. DOI: 10.13386/j.issn1002-0306.2013.22.061

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Optimization of activation condition of prothrombin from horse blood by using response surface methodology

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Based on single factor experiment, three main factors were screened out, including activation time, activation temperature, and concentration of Ca2 +. Optimization of these factors by using response surface methodology, the results showed that the optimum activation conditions of prothrombin were as follows:activation time 2.8h, temperature 32℃, concentration of Ca+20.11mol/L. Under these conditions, the specific activity of thrombin was 51.16U/mg.

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Science and Technology of Food Industry

Optimization of activation condition of prothrombin from horse blood by using response surface methodology

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