Phylogenetic analysis and growth characteristics of a moderate halophilic bacterium isolated from dried cuttlefish

A moderate halophilic bacterium was isolated from the surface of a commercial dried cuttlefish ( Sepia officinalis) product.The sequencing of the bacterial 16S rDNA and BLAST analysis suggested the fragment shares99% identity with that of some species of genera Halomonas and Salinicola.Phylogenetic analysis result showed the halophile and the four type strains of genus Salinicola clustered into a branch in the phylogenetic consensus tree.Therefore, the strain NYJ01 was a member of genus Salinicola, and a tentative name of Salinicola sp. NYJ01 was proposed.For the strain NYJ01, growth occurred at sodium chloride concentrations of 1% ²0% ( optimum at 3% ¹0%) , at 10~ 45℃ ( optimum at 25 ~ 37℃) . The strain grew well in mediums that consisted of 0.5% ~ 15% sodium nitrate, but it was not able to reduce nitrate into nitrite. Some food preservatives, e.g., 0.025% sodium diacetate, 0.05% potassium sorbate, 0.05% sodium benzoate or 0.05% sodium dehydroacetate can be used to inhibit growth of the strain NYJ01.