DNA extraction from soybean oil and detection of genetically-modified components by single-tube nested PCR

YAO Qin; SONG Hao; CHEN Feng

doi:10.13386/j.issn1002-0306.2013.21.027

YAO Qin, SONG Hao, CHEN Feng. DNA extraction from soybean oil and detection of genetically-modified components by single-tube nested PCR[J]. Science and Technology of Food Industry, 2013, (21): 183-186. DOI: 10.13386/j.issn1002-0306.2013.21.027

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DNA extraction from soybean oil and detection of genetically-modified components by single-tube nested PCR

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The two pairs of primers were synthesized according to genetically- modified soybean, located in the CaMV35s promoter, CTP gene, CP4EPSPS gene region, and detection method of transgenic factors by single-tube nested PCR was established.It was proved that DNA extraction by improved reagent kit method or freeze drying technology from soybean oil could meet the detection of single-tube nested PCR, and for single-tube nested PCR outer primers annealing temperature must be higher than inner primers.

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DNA extraction from soybean oil and detection of genetically-modified components by single-tube nested PCR

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