Optimization of initial fermentation media of coliform group by response surface methodology

LV Xiao-nan; TIAN Ran; JIANG Zhan-mei; ZHENG Dong-mei; HAN Jia-tong; TIAN Bo

doi:10.13386/j.issn1002-0306.2013.19.075

LV Xiao-nan, TIAN Ran, JIANG Zhan-mei, ZHENG Dong-mei, HAN Jia-tong, TIAN Bo. Optimization of initial fermentation media of coliform group by response surface methodology[J]. Science and Technology of Food Industry, 2013, (19): 131-134. DOI: 10.13386/j.issn1002-0306.2013.19.075

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Optimization of initial fermentation media of coliform group by response surface methodology

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The initial fermentation media of coliform group was optimized by single factor investigations, Box Behnken response surface methodology based on LST fermentation media.The influences of three factors: lactose, tryptone and yeast powder on the TVCs of coliform group were studied.Finally, the appropriate medium composition was lactose 0.5g/100mL, tryptone 1.64g/100mL, yeast powder 0.29g/100mL.Under these conditions, the TVCs of coliform group was 1.025 × 107cfu/mL.The actual value was 1.033 × 107cfu/mL through verification test.The actual value had no significant difference compared with the theoretical value.

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Optimization of initial fermentation media of coliform group by response surface methodology

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