Study on antioxidative activity and stability on the refined and high purity of anthocyanins of Lonicera edulis

The experiments studied antioxidation and stability of the refined and high purity anthocyanins from Lonicera edulis, in order to provide theoretical basis for its development and utilization.On the aspect of antioxidation, they were evaluated by scavenging capacity of·OH and DPPH·, the total reducing power, lipid peroxidation inhibition.The results showed that these two anthocyanins were stronger than that of VC on scavenging of DPPH· and lipid peroxidation inhibition, and the total reduction power and OH·scavenging capacity were weaker than that of VC but had a certain effect, and the high purity anthocyanin was better than the refined anthocyanin;on the aspect of stability, we studied on them by the effect of sunlight, several kinds of food additives, all kinds of metal ions, their oxidation and reducibility resistance, the results showed that the two anthocyanins have better preservation when the temperature was less than 60℃ and in dark;two anthocyanins were sensitive to both oxidizing and reducing agents, and had a certain resistance to oxidation but not strong reduction of resistance;two anthocyanins were relatively stable for the vast majority of metal ions, few metal ions such as Cu2 +, Fe2 +, Fe3 +had damage to them;NaCl, citric acid had the hyperchromic effect to these two anthocyanins, VC, potassium sorbate, sodium benzoate had hypochromic effect, sucrose had little effect;and high purity anthocyanin was worse than the refined anthocyanin on stability.To sum up, high purity anthocyanin had better antioxidant effect compared to the refined anthocyanin, but had worse stability than refined anthocyanin.