Optimization of purification technique of anthocyanin from Lonicera Edulis via response surface methodology (RSM)

Response surface methodology (RSM) was used to optimize purification of anthocyanin from Lonicera edulis.Based on single factor tests, three purification parameters including sample concentration, ratio of diameter to length, elution flow rate were optimized through Box-Benhnken central combination design and RSM for achieving maximum the purification of anthocyanin.The interaction of the respective variables and their influence on the purification were studied to obtain the optimum purification conditions of anthocyanin were as follows:the sample concentration 2.5mg/mL, diameter to length ratio 1∶25, eluent concentration 70% ethanol, elution velocity 3mL/min.Under such conditions, the purity of anthocyanins was up to 19.54%.