Study on the optimization of ethanol extraction of total flavonoids and alkaloids from lotus leaf using response surface methodology

The ethanol extraction process of lotus leaf total flavonoids and alkaloids was studied by ultrasonicenzyme-diacolation collaborative extraction technique.Box-Behnken response surface design method was used to investigate the effect of influence factors on overall desirability.The experimental data were analysed using second-order polynomial equation and 3D-response surface graphs.The optimum extraction conditions were as follows :ethanol concentration 75%, solid/liquid ratio 1∶26 (g/mL) , ultrasound time 80min, cellulase dosage 14mg/g, collecting 9 folds of percolate at a rate of 3mL/min, the proportion of total flavonoids and alkaloids was 6.7735% and 0.7817% under this condtions, respectively.The deviation between observed and predicted values of yield was-3%.The results indicated that the optimized extraction technique was a new method of higher efficiency and less time consumption.