Purification and hemagglutinating activity test of the lectin isolated from mycelium of Lyophyllum decastes from Qilian mountain

YANG Qiu-hui-zi; CHEN Yu-tai; ZHANG Zhi-kuan; WEI Sheng-long; DONG Ming-sheng

doi:10.13386/j.issn1002-0306.2013.18.007

YANG Qiu-hui-zi, CHEN Yu-tai, ZHANG Zhi-kuan, WEI Sheng-long, DONG Ming-sheng. Purification and hemagglutinating activity test of the lectin isolated from mycelium of Lyophyllum decastes from Qilian mountain[J]. Science and Technology of Food Industry, 2013, (18): 114-118. DOI: 10.13386/j.issn1002-0306.2013.18.007

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Purification and hemagglutinating activity test of the lectin isolated from mycelium of Lyophyllum decastes from Qilian mountain

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One novel lectin LML was purified from mycelium of Lyophyllum decastes using ion exchange chromatography and gel filtration.Based on the result of gel filtration and SDS-PAGE, LML was single subunit glycoprotein with a molecular mass of 48.2ku and having 10.36% neutral sugar.The hemagglutinating activity test showed that LML showed hemagglutinating activity on rabbit red blood cells.Its activity was stable in the pH range of 3 ¹0, temperature range of 20 ⁷0℃.The hemagglutinating activity of LML were inhibited by glucose (25mmol / L) and maltose (12.5mmol / L) , and were unaffected by Ca2+, Mg2+ and Zn2+ at LML concentrition≥250μg/mL.

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Science and Technology of Food Industry

Purification and hemagglutinating activity test of the lectin isolated from mycelium of Lyophyllum decastes from Qilian mountain

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