Study on antioxidant effect of polysaccharide from Citrus reticulata ‘Chachi' Peel

Objective:To study the protective effect of polysaccharide from Citrus reticulata ‘Chachi' Peel on H2O2-induced oxidative injury in PC12 cells.Methods:Cell viability was assayed by MTT method, the toxicity model was established by treating PC12 cells with H2O2.The level of MDA, SOD and GSH-Px were measured by spectroscopy, respectively.Results:After being exposed to 100μmol/L H2O2 for 4 hours, typical morphological characteristics of injury were observed.The level of MDA increased significantly, while that of SOD and GSHPx decreased significantly in H2O2- induced group compared with that of the sham group.Polysaccharide from Citrus reticulata ‘Chachi' Peel could decrease MDA content and increase SOD and GSH-Px activity significantly.Conclusions:Polysaccharide from Citrus reticulata ‘Chachi' Peel had protective effect on PC12 cells injury induced by H2O2 in culture.The protective effect might be related to the increased activity of antioxidant enzymes.