Preparation of microcapsules based on poly malic acid and gelatin

This paper described the preparation of novel biodegradable and edible nanocapsule based on self-assembly of β-Polymalic acid ( β-PMA) and gelatin at normal temperature and pressure.After the Plackett-Burman design ( PBD) by Design-Expert 7.0, it was found that the size and PDI of the nanocapsule measured by Malvern zetasizer Nano-ZS instrument depended on mass concentration of β-PMA and gelatin, pH of gelatin and reaction time.A Box-Behnken design ( BBD ) was used to optimize the preparation conditions of the β-PMA / gelatin nanocapsule, and then the best experimental condition was obtained as follows: the concentrations of β-PMA and gelatin were 0.1% and 0.13% , pH of gelatin was 4.0, reaction time was 25min. The size of the nanocapsules prepared under the best contidions was 288.37nm.