Characteristics of saccharide-protein linkage in the enzymatic hydrolysis of rice protein pretreated with high hydrostatic pressure

The binding characteristics between protein and saccharide of enzymatic hydrolysates of rice protein pretreated by 100MPa were researched. The results showed that the ratio of protein / saccharide in the soluble hydrolysates pretreated by 100MPa was lower than that of unpretreated.β-elimination reaction showed that protein and saccharide was linked together by N - glycoside peptide bond. FT - IR spectrum proved the insoluble hydrolyzed rice protein was yet glycoprotein. The staining with Coomassie brilliant blue and periodic acid - Schiff ( PAS) reaction in SDS-PAGE showed the 14ku subunit of insoluble hydrolyzed rice protein was yet glycoprotein. The results above indicated that high pressure treatment was helpful for the hydrolysis of rice glycoprotein, some of them was yet insoluble in water.