Preparation and the chromatographic behavior analysis of low degree polymerization of Chitooligosaccharides components

Low- degree polymerization chitooligosaccharides ( COS) were prepared with the chitosan hyrolized by chitosan enzymatic utilizing the ethanol precipitation method.A HPLC analysis method was also presented for COS with remarkable separation and high sensitivity based on Shodex NH2P-50 4E Column and acetonitrile- ammonia mobile phase as the gradient elution.The N-acetylated constituent in COS could be identified using UPLC-MS.The chromatographic behavior of COS was also studied. Results illustrated that the N- acetylated constituent in COS was the main reason for that chromatographic separation of chitosan is not in accordance with its polymerization degree, which was very important for analyzing and separating the constituents of chitosan.