Optimization of process conditions for tilapia skin collagen peptides chelating with magnesium by response surface methodology

With Tilapia skin as material, chelating conditions for skin collagen peptides and magnesium ( II) iron were investigated.The effects of collagen peptides - magnesium chloride ratio, pH, tempretureand reaction time on chelating efficiency were discussed. According to single test and response surface experiment, the optimum preparation conditions of collagen peptides - magnesium chloride were obtained as follows: collagen peptides / magnesium mass ratio of 1∶5, pH7.0, temperature 70℃, reaction time 30min.Under these conditions, the chelation rate was up to 84.58%.The results from fourier transform infrared spectroscopy scan also proved that the collagen peptides of Tilapia fish skin and magnesium ions were chaleted and turned into a new kind of chelate compoud.