Comparison of similarity of H+-ATPase coding gene between wild strain and mutant strain with low post-acidification of Lactobacillus delbrueckii subsp.bulgaricus

In order to determine the regulation mechanism of H + - ATPase of Lactobacillus delbrueckii subsp. bulgaricus, a H + - ATPase deficiency strain Lactobacillus delbrueckii subsp. bulgaricus KLDS1.9201 - 11 with low post - acidification which was derived from Lactobacillus delbrueckii subsp. bulgaricus KLDS1.9201 in previous research and the wild type strain KLDS1.9201 were investigated.The genomic DNA was extracted from KLDS1.9201 -11 and KLDS1.9201 respectively, and the coding genes of H+-ATPase of these two strains were acquired by PCR amplification, then PCR products were sequenced.At last the similarity of coding genes of H+-ATPase between the KLDS1.9201-11 and KLDS1.9201 was compared by the software of DNAMAN.Results showed that the similarity of coding genes of H+-ATPase between the KLDS1.9201-11 and KLDS1.9201 was 100%.In conclusion the differential of H+-ATPase activity of KLDS1.9201-11 and KLDS1.9201 was not caused by the mutation of coding gene of H + - ATPase.And it might the lower expression of H+-ATPase of mutant strain KLDS1.9201-11 which in turn caused the reduced H+-ATPase activity.It provided the theoretical basis for further investigating the regulation mechanism of H+-ATPase of Lactobacillus delbrueckii subsp.bulgaricus.