Extraction of dietary fiber from Ganoderma atrum with response surface optimization

Response surface methodology was applied to optimize the extraction conditions ofsoluble dietary fiber (SDF) and insoluble dietary fiber (IDF) from Ganoderma atrum.Effects of enzymolysis temperature, reaction time and material-liquid ratio on the yield of SDF and alkaline extraction pH, temperature and time on the yield of IDF were investigated.Results exhibited that the optimum extraction conditions were as follows:enzymolysis temperature and reaction time was 98℃ and 103min, solid-liquid ratio was 1∶32, alkaline extraction pH, temperature and time was 10, 48℃, 41min.The yield of SDF and IDF was 1.08% and 88.68%, respectively, under optimized conditions.