Characteristics of the sublethal injured and resuscitative Vibrio parahaemolyticus

Objective:In order to research the characteristics of the sublethal injured and the resuscitative Vibrio parahaemolyticus, the morhphological characteristics and the virulence genes were investigated.Methods:The growth curses of sublethal injured and resuscitative Vibrio parahaemolyticus BJ1.1616 and BJ1.1997 were made, and then the cellular materials leakage of Vp was determined.The morphological characteristics of the sublethal injured and the resuscitative strains were observed by using SEM, and the physiological and biochemical characteristics and the virulence genes were detected.Results:The injured strains showed an extended lag phase, but the growth curses of the resuscitative strains were similar with the normal strains.The supernatant of the cell suspension after thermal treatment showed a significantly (p<0.05) higher absorbance at 260 and 280nm when compared with that of the control cells, but the resuscitative cell did not differ significantly (p>0.05) .There was no difference between VpBJ1.1616 and normal bacteria in 260nm and 280nm absorbing materials.Compared with the normal strains, the morphological characteristics of sublethal injured and resuscitative strains and some of the physiological and biochemical characteristics changed significantly and irreversible, but the virulence genes remained.Conclusion:These data of sublethal injury and resuscitative conditions can provided a basis on the mechanism study, as well as the detection of sublethal injury Vp.